Research Paper Volume 13, Issue 1 pp 865—876

Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis

Propofol suppresses the rI/R-induced release of pyroptotic proteins and inflammatory cytokines in the lungs of rats. Rats were subjected to rI/R, with or without propofol (5 or 10 mg/kg) treatment for 24 h. (A–C) Immunofluorescence analyses were used to measure the fluorescence intensity levels of NLRP3, ASC and caspase 1. (D, E) ELISAs were used to measure the protein levels of IL-1β and IL-18 (*P #P

Figure 5. Propofol suppresses the rI/R-induced release of pyroptotic proteins and inflammatory cytokines in the lungs of rats. Rats were subjected to rI/R, with or without propofol (5 or 10 mg/kg) treatment for 24 h. (AC) Immunofluorescence analyses were used to measure the fluorescence intensity levels of NLRP3, ASC and caspase 1. (D, E) ELISAs were used to measure the protein levels of IL-1β and IL-18 (*P < 0.05 vs. sham; #P < 0.05 vs. rI/R).