Research Paper Volume 13, Issue 1 pp 865—876

Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis

The inhibition of SIRT1 prevents propofol from ameliorating the morphological characteristics of ALI and suppressing pyroptosis in the lungs of rI/R rats. Rats were subjected to rI/R, with or without propofol (10 mg/kg) and/or nicotinamide (60 mg/kg) treatment for 24 h. (A) H and E analysis was performed, and the lung injury score was determined. (B) Immunohistochemistry was used to measure the fluorescence intensity of myeloperoxidase. (C) Immunofluorescence analysis was used to measure the fluorescence intensity of F4/80. (D) TUNEL analysis was used to measure the apoptotic index (%). (E–G) Immunofluorescence analyses were used to measure the fluorescence intensity levels of NLRP3, ASC and caspase 1. (H, I) ELISAs were used to measure the protein levels of IL-1β and IL-18 (*P #P $P

Figure 7. The inhibition of SIRT1 prevents propofol from ameliorating the morphological characteristics of ALI and suppressing pyroptosis in the lungs of rI/R rats. Rats were subjected to rI/R, with or without propofol (10 mg/kg) and/or nicotinamide (60 mg/kg) treatment for 24 h. (A) H and E analysis was performed, and the lung injury score was determined. (B) Immunohistochemistry was used to measure the fluorescence intensity of myeloperoxidase. (C) Immunofluorescence analysis was used to measure the fluorescence intensity of F4/80. (D) TUNEL analysis was used to measure the apoptotic index (%). (EG) Immunofluorescence analyses were used to measure the fluorescence intensity levels of NLRP3, ASC and caspase 1. (H, I) ELISAs were used to measure the protein levels of IL-1β and IL-18 (*P < 0.05 vs. sham; #P < 0.05 vs. rI/R; $P < 0.05 vs. rI/R + propofol).