Figure 6. Coicis semen treatment activated the TGF-β1-ALK1-Smad1/5 signaling pathway after stroke. (A) Western blot showing that Coicis Semen treatment increased the expression and phosphorylation of the TGF-β1-ALK1-Smad1/5-associated proteins TGF-β1, ALK-1, Smad 1/5, p-Smad 1/5 and CD31 at 7 and 14 days after stroke. (B–E) Quantitative analysis of the protein levels of TGF-β1, ALK-1, p-Smad 1/5 and CD31 in ischemic cortical tissue. Mean ± SD. n = 5. aP < 0.05 vs. vehicle. Statistical analyses were performed by two-tailed Student’s t-test.