Research Paper Volume 13, Issue 1 pp 910—932

Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

Effects of Tan IIA on HCh diet-induced AS lesion formation, serum lipid levels and cytokine productions in ApoE-/- mice. (H–I) Levels of LDL-Ch and TG in the sera of ApoE-deficient mice fed with different diets for 16 weeks. Oxidized LDL-induced and Tan IIA inhibited cytokine (IL-1β, TNFα, IL-6 and IL-18) productions at mRNA levels measured by qRT-PCR in the aortic tissues (J–M) and at protein levels determined by ELISA in the sera (N–Q). (R, S) Effects of Tan IIA (2.5, 5, 10 μg/mL) on oxLDL-induced IL-1β productions in the culture media of LPS-primed and un-primed mouse B6 macrophages, respectively. (T, U) Effects of Tan IIA on oxLDL-induced caspase-1 activation (10 kDa) and IL-1β maturation (17 kDa) in mouse B6 macrophages.

Figure 1. Effects of Tan IIA on HCh diet-induced AS lesion formation, serum lipid levels and cytokine productions in ApoE-/- mice. (HI) Levels of LDL-Ch and TG in the sera of ApoE-deficient mice fed with different diets for 16 weeks. Oxidized LDL-induced and Tan IIA inhibited cytokine (IL-1β, TNFα, IL-6 and IL-18) productions at mRNA levels measured by qRT-PCR in the aortic tissues (JM) and at protein levels determined by ELISA in the sera (NQ). (R, S) Effects of Tan IIA (2.5, 5, 10 μg/mL) on oxLDL-induced IL-1β productions in the culture media of LPS-primed and un-primed mouse B6 macrophages, respectively. (T, U) Effects of Tan IIA on oxLDL-induced caspase-1 activation (10 kDa) and IL-1β maturation (17 kDa) in mouse B6 macrophages.