Research Paper Volume 13, Issue 1 pp 933—943

IRF-1 contributes to the pathological phenotype of VSMCs during atherogenesis by increasing CCL19 transcription

Effects of silencing CCL19 on VSMCS. Detection of silencing efficiency of sh-CCL19 plasmid was detected by qRT-PCR. (A) Knockdown of CCL19 inhibited mRNA level of IL1-α (B), IL-1β (C), TNF-α (D), IL-6 (E) and collagen1 (F), collagen3 (G) and OPN (H) induced by PDGF-BB.n=5 (I) Western blot show collagen1 and IL-6 protein expression. n = 4. Migration (J) and proliferation (K) of VSMCS after silencing CCL19. n = 6. *P

Figure 3. Effects of silencing CCL19 on VSMCS. Detection of silencing efficiency of sh-CCL19 plasmid was detected by qRT-PCR. (A) Knockdown of CCL19 inhibited mRNA level of IL1-α (B), IL-1β (C), TNF-α (D), IL-6 (E) and collagen1 (F), collagen3 (G) and OPN (H) induced by PDGF-BB.n=5 (I) Western blot show collagen1 and IL-6 protein expression. n = 4. Migration (J) and proliferation (K) of VSMCS after silencing CCL19. n = 6. *P<0.05; **P<0.01.