Research Paper Volume 13, Issue 1 pp 957—972

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells

Inhibition of Hif-1 suppressed increased expression of Bnip3 in activated hepatic stellate cells. Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days and Hif-1 chemical inhibitor YC-1 (50 μM) was added as cells were cultivated to 2 days. (A) Cell lysates were subjected to detect Hif-1α and Bnip3 with Western blot. Densitometric analysis was performed and data were expressed as mean ± SD, *P P P B) Immunofluorescence assay was performed to detect Bnip3 (Cy3) by confocal microscopy. (C) LX-2 cells were stimulated by 100 μM CoCl2 or 2 μg/ml LPS either alone or after YC-1 pre-treatment (50 μM). Cell lysates were subjected to detect Hif-1α and Bnip3 with Western blot. Densitometric analysis was performed and data were expressed as mean ± SD, *P P P D) Immunofluorescence assay was performed to detect Bnip3 (Cy3) by confocal microscopy.

Figure 4. Inhibition of Hif-1 suppressed increased expression of Bnip3 in activated hepatic stellate cells. Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days and Hif-1 chemical inhibitor YC-1 (50 μM) was added as cells were cultivated to 2 days. (A) Cell lysates were subjected to detect Hif-1α and Bnip3 with Western blot. Densitometric analysis was performed and data were expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. (B) Immunofluorescence assay was performed to detect Bnip3 (Cy3) by confocal microscopy. (C) LX-2 cells were stimulated by 100 μM CoCl2 or 2 μg/ml LPS either alone or after YC-1 pre-treatment (50 μM). Cell lysates were subjected to detect Hif-1α and Bnip3 with Western blot. Densitometric analysis was performed and data were expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. (D) Immunofluorescence assay was performed to detect Bnip3 (Cy3) by confocal microscopy.