Research Paper Volume 13, Issue 1 pp 957—972

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells

Bnip3 interacted with vimentin in activated hepatic stellate cells. LX-2 cells were treated with 100 μM CoCl2 or 2 μg/ml LPS for 8 h. Culture-activated primary HSCs from mice were cultured up to 2 days or 6 days. (A) 300μg cell lysates of LX-2 cells and 150μg cell lysates of primary HSCs from mice were collected and immunoprecipitated with anti-Bnip3, followed by immunoblotting with anti-vimentin, anti-phosphorylated vimentin S56, S72, S82 and S38. (B, C) Immunofluorescence assay was performed to detect Bnip3 (Cy3) and vimentin (FITC) in LX-2 cells (B) and culture-activated primary HSCs from mice (C) by confocal microscopy.

Figure 6. Bnip3 interacted with vimentin in activated hepatic stellate cells. LX-2 cells were treated with 100 μM CoCl2 or 2 μg/ml LPS for 8 h. Culture-activated primary HSCs from mice were cultured up to 2 days or 6 days. (A) 300μg cell lysates of LX-2 cells and 150μg cell lysates of primary HSCs from mice were collected and immunoprecipitated with anti-Bnip3, followed by immunoblotting with anti-vimentin, anti-phosphorylated vimentin S56, S72, S82 and S38. (B, C) Immunofluorescence assay was performed to detect Bnip3 (Cy3) and vimentin (FITC) in LX-2 cells (B) and culture-activated primary HSCs from mice (C) by confocal microscopy.