Research Paper Volume 12, Issue 24 pp 25981—25999

Profilin 2 promotes growth, metastasis, and angiogenesis of small cell lung cancer through cancer-derived exosomes

Figure 5. Exosomal PFN2 from H446 cells affect migration and tube formation ability of HUVEC cells. Co-culturing with H446-OE conditioned medium promoted (A) migration (P=0.0022) and (B) tube formation ability (P=0.0255) of HUVEC cells. Co-culturing with H446-KD cells conditioned medium remarkably decreased the migration (P=0.0319) (C) and tube formation ability (P=0.0017) (D) of HUVEC cells. When the exosomes are removed from the CM, PFN2 expression in the CM is significantly decreased (P=0.0267) (E). Exosome depletion from the CM weakens the migration (P=0.035) (F) and tube formation ability (P=0.0112) (G) of HUVEC cells. Exosomes derived from H446 cells could be internalized in HUVEC cells (H). OE-exo elevated the migration (P=0.02) (I) and tube length (P=0.0029) (J) of HUVEC cells. However, KD-exo inhibited the migration (P=0.0093) (K) and tube length (P=0.03) (L) of HUVEC cells.