Research Paper Volume 13, Issue 3 pp 3368—3385

Amorphous nano-selenium quantum dots prevent pulmonary arterial hypertension through recoupling endothelial nitric oxide synthase

Administration of A-SeQDs improves pulmonary arterial remodeling in monocrotaline-injected mice. The experimental protocol was shown in Supplementary Figure 3A. At the end of experiment, mice were sacrificed under anesthesia. (A) The hearts were isolated to measure the ratio of right ventricle (RV) to left ventricle (LV) plus septum (S) weights [RV/(LV+S)]. (B and C) Pulmonary arteries were subjected to perform HE staining and sirius red staining in B. The thickness of pulmonary artery was calculated in C. All data were expressed as mean ± SEM. 10-15 mice were in each group. *PVS Vehicle alone, #PVS monocrotaline alone. &PVS monocrotaline plus Selenium or C-SeQDs. (D) The content of selenium in lung tissue was determined. *PVS Vehicle or monocrotaline alone, #PVS. monocrotaline plus Selenium or C-SeQDs. A one-way ANOVA followed by Tukey post-hoc tests was used to produce the P values.

Figure 3. Administration of A-SeQDs improves pulmonary arterial remodeling in monocrotaline-injected mice. The experimental protocol was shown in Supplementary Figure 3A. At the end of experiment, mice were sacrificed under anesthesia. (A) The hearts were isolated to measure the ratio of right ventricle (RV) to left ventricle (LV) plus septum (S) weights [RV/(LV+S)]. (B and C) Pulmonary arteries were subjected to perform HE staining and sirius red staining in B. The thickness of pulmonary artery was calculated in C. All data were expressed as mean ± SEM. 10-15 mice were in each group. *P<0.05 VS Vehicle alone, #P<0.05 VS monocrotaline alone. &P<0.05 VS monocrotaline plus Selenium or C-SeQDs. (D) The content of selenium in lung tissue was determined. *P<0.05 VS Vehicle or monocrotaline alone, #P<0.05 VS. monocrotaline plus Selenium or C-SeQDs. A one-way ANOVA followed by Tukey post-hoc tests was used to produce the P values.