Figure 6. A-SeQDs-induced alleviation of PAH is DHFR dependent in monocrotaline-injected mice. The experimental protocol was shown in Supplementary Figure 3A. Wildtype (WT) and DHFR-/- mice were given A-SeQDs administration in regular diet one week prior to a single intraperitoneal injection of 100 mg/kg monocrotaline. Three weeks after injection, right ventricle systolic pressure (RVSP) in (A) and the ratio of right ventricle (RV) to left ventricle (LV) plus septum (S) weights [RV/(LV+S)] in (B) were measured. Pulmonary arteries isolated from mice were subjected to perform HE staining and sirius red staining in (C) and the thickness of pulmonary artery was calculated in (D). All data were expressed as mean ± SEM. 10-15 mice were in each group. *P<0.05 VSWT alone. A one-way ANOVA followed by Tukey post-hoc tests was used to produce the P values.