Research Paper Volume 13, Issue 1 pp 973—990

Tandem mass tag-based proteomic analysis reveals cathepsin-mediated anti-autophagic and pro-apoptotic effects under proliferative diabetic retinopathy

The expression of CTSB, CTSD, and CTSL in retinal vascular endothelial cells treated with high glucose. (A–F) The immunofluorescence of CTSB, CTSD, and CTSL proteins in the HG-treated simian retinal vascular endothelial cells (RF/6A) and normal controls. Scale bar = 100 μm (G) The concentrations of CTSB, CTSD, and CTSL proteins in RF/6A cell culture supernatant. (H–J) The relative expression levels of CTSB, CTSD, and CTSL genes in HG-stimulated and normal RF/6A cells. n = 6 / group. Data are expressed as mean ± SEM. * P P

Figure 5. The expression of CTSB, CTSD, and CTSL in retinal vascular endothelial cells treated with high glucose. (AF) The immunofluorescence of CTSB, CTSD, and CTSL proteins in the HG-treated simian retinal vascular endothelial cells (RF/6A) and normal controls. Scale bar = 100 μm (G) The concentrations of CTSB, CTSD, and CTSL proteins in RF/6A cell culture supernatant. (HJ) The relative expression levels of CTSB, CTSD, and CTSL genes in HG-stimulated and normal RF/6A cells. n = 6 / group. Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01. HG: high glucose.