Research Paper Volume 12, Issue 24 pp 26000—26011

Suppression of long noncoding RNA LINC00324 restricts cell proliferation and invasion of papillary thyroid carcinoma through downregulation of TRIM29 via upregulating microRNA-195-5p

Figure 3. miR-195-5p interacts with LINC00324 in PTC cells. (A) Sequence alignment between miR-195-5p and WT or MT LINC00324. (B) A dual-luciferase reporter assay was carried out using 293T cells cotransfected with WT or MT LINC00324 reporter vectors and miR-195-5p mimic. Relative levels of LINC00324 (C) input and (D) pulled-down in RNA pull-down assay. An RNA pull-down assay was carried out using biotinylated WT or MT miR-195-5p mimic in TPC1 and K1 cells. LINC00324 expression in RNA precipitates was examined by RT-qPCR. (E) Levels of lINC00324 in TPC1 and K1 cells transfected with LINC00324 siRNA or control siRNA were assessed via RT-qPCR. *p < 0.05.