Research Paper Volume 12, Issue 24 pp 26000—26011

Suppression of long noncoding RNA LINC00324 restricts cell proliferation and invasion of papillary thyroid carcinoma through downregulation of TRIM29 via upregulating microRNA-195-5p

Figure 4. miR-195-5p contributes to LINC00324-mediated effects in PTC cells. (A) Levels of miR-195-5p in PTC tissue and normal tissue were examined via RT-qPCR. ****p < 0.0001 versus normal. (B) miR-195-5p was inversely associated with LINC00324 in PTC tissue (r=-0.8715, p<0.0001). (C) Levels of miR-195-5p in PTC cell lines (B-CPAP, KTC-1, TPC1, and K1) and Nthy-Ori 3-1 cells were measured via RT-qPCR. *p < 0.05 versus Nthy-Ori 3-1. (D, E)Effect of miR-195-5p overexpression on cellular proliferation of TPC1 and K1 cells was assessed via a CCK-8 assay. (F, G) Effect of miR-195-5p overexpression on the invasive potential of TPC1 and K1 cells was evaluated by a transwell invasion assay. *p < 0.05 versus NC mimic. TPC1 and K1 cells were transfected with LINC00324 siRNA and miR-195-5p inhibitor for 48 h, and (H, I) cellular proliferation was evaluated via a CCK-8 assay, and (J, K) cellular invasion was monitored via a transwell invasion assay. *p < 0.05.