Figure 5. miR-195-5p negatively modulates TRIM29 expression. (A) Sequence alignment between miR-195-5p and WT or MT TRIM29 3′-UTR. (B) A dual-luciferase reporter assay was carried out using 293T cells cotransfected with WT or MT TRIM29 3′-UTR reporter vectors and miR-195-5p mimic. TPC1 and K1 cells were transfected with miR-195-5p mimic, miR-195 inhibitor, or NC controls for 48 h. (C) Levels of TRIM29 mRNA were determined via RT-qPCR. (D) Levels of TRIM29 protein were evaluated via western blot. *p < 0.05.