Figure 6. The effects of Wntless deletion in granulosa cells on ovulation and the expression of β-Catenin and LHCGR. (A) The number of ovulated oocytes in control (n=7) and mutant mice (n=6) after superovulation. (B) Relative mRNA levels of ovulation related genes in ovaries at 8 h post-hCG treatment. (C) The levels of β-Catenin and LHCGR in ovaries at 48 h post-hCG treatment measured by western blot. The β-Catenin and LHCGR levels were normalized to GAPDH. (D) Immunostaining of β-Catenin in the ovary at 8 h post-hCG treatment. Scale bar =100 μm. DNA, blue; β-Catenin, yellow. In (A–C), data are shown as the mean ± SEM. Experiments were replicated a minimum of 4 times.