Research Paper Volume 13, Issue 1 pp 1096—1119

The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles

The activation of PFs by eNOS depended on cGMP/PKG. (A, B) Ovaries at 1 dpp were treated with the vehicle, SN (100 μM), ODQ (1 μM), SN + ODQ, NM (1 mM), YC (10 μM) or NM + YC for six days (n=6). (A) The ovarian morphology was analyzed after hematoxylin staining. (B) The numbers of PFs and GFs/the total number of follicles were analyzed. (C, D) Ovaries at 1 dpp were treated with the vehicle, YC (10 μM), KT (1 μM), YC + KT, ODQ (1 μM), 8Br (10 μM) or ODQ + 8Br for six days (n=6). (C) The ovarian morphology and (D) the numbers of PFs and GFs/the total number of follicles were analyzed. Scale bar, 40 μm. Different letters with the same color denote statistical significance at p

Figure 3. The activation of PFs by eNOS depended on cGMP/PKG. (A, B) Ovaries at 1 dpp were treated with the vehicle, SN (100 μM), ODQ (1 μM), SN + ODQ, NM (1 mM), YC (10 μM) or NM + YC for six days (n=6). (A) The ovarian morphology was analyzed after hematoxylin staining. (B) The numbers of PFs and GFs/the total number of follicles were analyzed. (C, D) Ovaries at 1 dpp were treated with the vehicle, YC (10 μM), KT (1 μM), YC + KT, ODQ (1 μM), 8Br (10 μM) or ODQ + 8Br for six days (n=6). (C) The ovarian morphology and (D) the numbers of PFs and GFs/the total number of follicles were analyzed. Scale bar, 40 μm. Different letters with the same color denote statistical significance at p < 0.01 (Red letters represent the proportions of PFs, while black letters represent the proportions of GFs).