**Figure 5.** **The eNOS/cGMP/PKG pathway activated PFs via mTOR in preGCs.** (**A**) The relative mRNA levels of mTOR in oocytes and somatic cells isolated from ovaries at 3 dpp. *** denotes statistical significance at *p* < 0.001. (**B**) The colocalization of mTOR and PKG was detected via immunofluorescence in neonatal ovaries at 3 and 7 dpp. Arrows indicate PFs and triangles indicate GFs. Scale bar, 40 μm. Ovaries at 1 dpp were treated with the vehicle, 8Br (10 μM), the mTOR inhibitor rapamycin (Rapa) or 8Br + Rapa for six days (n=6). (**C**) The ovarian morphology and 9 (**D**) the numbers of PFs and GFs/the total number of follicles were analyzed. Scale bar, 40 μm. (**E**) Ovaries at 1 dpp were treated with the vehicle, 8Br (10 μM), the KIT inhibitor AX (5 μM) or 8Br + AX for six days (n=6). The numbers of PFs and GFs/the total number of follicles were analyzed. Different letters with the same color denote statistical significance at *p* < 0.001 (Red letters represent the proportions of PFs, while black letters represent the proportions of GFs).