**Figure 8.** **The eNOS/cGMP/PKG pathway activated PFs by inhibiting FBXW7-induced mTOR ubiquitination.** (**A**–**C**) Six days after transfection, (**A**) the ovarian morphology, (**B**) the total number of oocytes and (**C**) the numbers of PFs and GFs/the total number of follicles were analyzed (n=8). Scale bar, 40 μm. (**D**) Immunofluorescence staining of FOXO3a in the empty vector and OE vector groups. Scale bar, 40 μm. (**E**) The number of oocytes with CL-FOXO3a/the total number of oocytes was analyzed in each group (n=8). (**F**) The number of PFs with CL-FOXO3a/the total number of PFs was analyzed in each group (n=8). (**G**) The negative correlation between the relative mRNA levels of FBXW7 and PKG. (**H**) The negative correlation between the relative mRNA levels of FBXW7 and the levels of cGMP. (**I**) The relative mRNA levels of FBXW7 in ovaries treated with the vehicle, SN, NM, 8Br or KT. *, ** and *** denote statistical significance at *p* < 0.05, *p* < 0.01 and *p* < 0.001, respectively. (**J, K**) After treatment with the empty vector, empty vector + SN, OE vector or OE vector + SN for six days, (**J**) the ovarian morphology, and (**K**) the numbers of PFs and GFs/the total number of follicles were analyzed (n=8). Scale bar, 40 μm. Different letters with the same color denote statistical significance at *p* < 0.01 (Red letters represent the proportions of PFs, while black letters represent the proportions of GFs).