Figure 5. RG1-MSC-CM downregulates inflammatory responses induced by radiation in vivo and in vitro. (A) Pro/anti-inflammatory cytokines were extracted from jejunal protein of irradiated rats on day 3. The level of cytokines was determined by enzyme-linked immunosorbent assay (ELISA). (3~4 rats/group). **, P < 0.05 versus IR + DMEM-F12. ##, P < 0.05 versus IR + MSC-CM. (B) Pro/anti-inflammatory cytokines of irradiated IEC-6 was measured by ELISA on day 3 after radiation. Data represent mean ± SD (n=3). ##, P < 0.05 versus IR + MSC-CM. **, P < 0.05 versus IR + DMEM-F12. (C) The percentages of CD4+Foxp3+ Treg cells in the CD4+ population of mesenteric lymph nodes (MLNs) were determined by flow cytometry (4~5 rats/group). Tissue samples were collected on day 3 of experiment. Data represent the mean ± SD. ##, P < 0.05 versus IR + DMEM-F12. All data were analyzed by t-test or one-way ANOVA except as otherwise indicated.