Figure 7. RG1-MSC-CM improves regeneration of intestinal stem cells (ISCs) in the irradiated rats. (A) Immunofluorescence staining with Lgr5. Intestinal samples were collected and stained on day 3 after radiation. Arrowheads indicate Lgr5-positive cells. Scale bars 50 μm. (B) Quantification of Lgr5-positive cells. n = 3~5 in each group. The number of positive cells in 5 crypts was scored in 100 crypts per section and reported as mean ± SD. **, P < 0.05 versus IR + DMEM-F12. ##, P < 0.05 versus IR + MSC-CM. All data were analyzed by t-test or one-way ANOVA except as otherwise indicated.