Research Paper Advance Articles

Exosomes derived from BDNF-expressing 293T attenuate ischemic retinal injury in vitro and in vivo

Caveolin-1 was involved in the endocytosis of 293T-Exo by R28 cells. The colocation of 293T-Exo with caveolin-1, but not clathrin, was observed using the fluorescence assay. The nuclei were stained with DAPI (blue), 293T-Exo was stained with fluorescent-tag (green), and the caveolin-1 or clathrin was stained with corresponding antibodies (red), respectively. Scale bar = 20 μm.

Figure 5. Caveolin-1 was involved in the endocytosis of 293T-Exo by R28 cells. The colocation of 293T-Exo with caveolin-1, but not clathrin, was observed using the fluorescence assay. The nuclei were stained with DAPI (blue), 293T-Exo was stained with fluorescent-tag (green), and the caveolin-1 or clathrin was stained with corresponding antibodies (red), respectively. Scale bar = 20 μm.