Figure 6. Caveolin-1-mediated endocytosis of 293T-Exo was inhibited by MBCD. (A) Pretreatment of MBCD in R28 cells inhibited the endocytosis of 293T-Exo, as determined by the fluorescence assay. The nuclei were stained with DAPI (blue), the cytoskeleton was stained with tubulin (red), and 293T-Exo was stained with fluorescent-tag (green). Scale bar = 20 μm. (B) Efficacy of endocytosis of 293T-Exo by R28 cells was reversed due to increasing doses of MBCD. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.