Research Paper Volume 12, Issue 24 pp 26029—26046

DUSP5 suppresses interleukin-1β-induced chondrocyte inflammation and ameliorates osteoarthritis in rats

Figure 5. Effect of DUSP5 knockdown on IL-1β-induced NF-κB and ERK signaling pathways in chondrocytes. (A, B) The protein expression and quantitation of DUSP5, p-p65, and p-ERK. GAPDH was used as the control. (C) The nuclear translocation of p65 was detected by immunofluorescence; DAPI was used to stain the DNA. Blue, DAPI; red, p65. (D) Quantitation of immunofluorescence staining of p65. DUSP5 knockdown chondrocytes were incubated with IL-1β (10 ng/mL) for 10 min. All data are expressed as mean±S.D. (n = 3). *p<0.05, **p<0.01.