Research Paper Volume 13, Issue 1 pp 1186—1211

MicroRNA-139-5p upregulation is associated with diabetic endothelial cell dysfunction by targeting c-jun

The molecular changes in HLI diabetic mice after injecting miR-139-5p antagomir or ECFCs. (A) Representative fluorescent images and quantitation of CM-DiI-labeled ECFCs (red, white arrow) and alpha smooth muscle actin (α-SMA)-positive arteriole in the ischemic adductor muscle tissue (green), as well as the merged images of CM-DiI, α-SMA, and DAPI. The representative images of arteriogenesis were expressed as α-SMA-positive arteriole area under 5 high-power fields (200X) by PBS control. (N=3) *PB) C-jun, PDGF-B, and VEGF expression in gastrocnemius muscle of ischemic lower extremity. (N=3) *PC) MiR-139-5p expression in gastrocnemius muscle of ischemic lower extremity. (N=3) *P

Figure 11. The molecular changes in HLI diabetic mice after injecting miR-139-5p antagomir or ECFCs. (A) Representative fluorescent images and quantitation of CM-DiI-labeled ECFCs (red, white arrow) and alpha smooth muscle actin (α-SMA)-positive arteriole in the ischemic adductor muscle tissue (green), as well as the merged images of CM-DiI, α-SMA, and DAPI. The representative images of arteriogenesis were expressed as α-SMA-positive arteriole area under 5 high-power fields (200X) by PBS control. (N=3) *P<.05 versus ECFCs transplantation alone group. (B) C-jun, PDGF-B, and VEGF expression in gastrocnemius muscle of ischemic lower extremity. (N=3) *P<.05 versus PBS or ECFCs. Data shown in all the graphs represent mean ± standard deviation. (C) MiR-139-5p expression in gastrocnemius muscle of ischemic lower extremity. (N=3) *P<.05 versus PBS. Data shown in the graphs represent mean ± standard deviation.