Research Paper Volume 12, Issue 24 pp 24651—24670

Downregulated genes by silencing MYC pathway identified with RNA-SEQ analysis as potential prognostic biomarkers in gastric adenocarcinoma

Figure 5. Quantification of TTLL12 (A), CDC16 (B) and UBE2T (C) mRNA and protein levels by tumor stage in GC of the diffuse-type. *p <0.008, **p <0.001, ***p <0.0002, ****p <0.0001, significant difference between groups (Nonparametric Mann – Whitney test). In all graphs, the expression in gastric tumors was normalized by matched non-neoplastic gastric tissue. RQ: relative quantification; T: tumor sample; N: normal mucosa sample. (D) ROC curve analysis. The cutoff point was chosen as the highest point of the Area-Under-the-Curve (AUC) for TTLL12, AUC: 0.713; (95% CI 0.62-0.76) with 76.02% sensitivity and 50.04% specificity, for CDC16, AUC: 0.812; (95% CI 0.70-0.83) with 85.0% sensitivity and 50.08% specificity and for UBE2T, AUC: 0.715; (95% CI 0.63-0.77) with 77.06% sensitivity and 48.03% specificity. (E) Heat maps showing the average levels of gene expression of ~35 samples of GC tissues of the diffuse-type. The red gradient shows the highest levels of expression while the blue gradient shows the lowest levels. Kaplan-Meier analysis of the overall survival (in months) of patients with diffuse gastric cancer as a function of (F) TTLL12, (G) CDC16 and (H) UBE2T expression. We analyzed high expression (gene expression ≥1.5; red line), as opposed to low expression (gene expression <1.5; blue line) associated with a higher probability of survival. We found more significant values when we associated the high expression of CDC16 (p <0.0001) and UBE2T (p = 0.0024), with patients who had a shorter survival.