Figure 5. Quantification of TTLL12 (A), CDC16 (B) and UBE2T (C) mRNA and protein levels by tumor stage in GC of the diffuse-type. *p <0.008, **p <0.001, ***p <0.0002, ****p <0.0001, significant difference between groups (Nonparametric Mann – Whitney test). In all graphs, the expression in gastric tumors was normalized by matched non-neoplastic gastric tissue. RQ: relative quantification; T: tumor sample; N: normal mucosa sample. (D) ROC curve analysis. The cutoff point was chosen as the highest point of the Area-Under-the-Curve (AUC) for TTLL12, AUC: 0.713; (95% CI 0.62-0.76) with 76.02% sensitivity and 50.04% specificity, for CDC16, AUC: 0.812; (95% CI 0.70-0.83) with 85.0% sensitivity and 50.08% specificity and for UBE2T, AUC: 0.715; (95% CI 0.63-0.77) with 77.06% sensitivity and 48.03% specificity. (E) Heat maps showing the average levels of gene expression of ~35 samples of GC tissues of the diffuse-type. The red gradient shows the highest levels of expression while the blue gradient shows the lowest levels. Kaplan-Meier analysis of the overall survival (in months) of patients with diffuse gastric cancer as a function of (F) TTLL12, (G) CDC16 and (H) UBE2T expression. We analyzed high expression (gene expression ≥1.5; red line), as opposed to low expression (gene expression <1.5; blue line) associated with a higher probability of survival. We found more significant values when we associated the high expression of CDC16 (p <0.0001) and UBE2T (p = 0.0024), with patients who had a shorter survival.