Research Paper Volume 12, Issue 24 pp 24651—24670

Downregulated genes by silencing MYC pathway identified with RNA-SEQ analysis as potential prognostic biomarkers in gastric adenocarcinoma

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Figure 6.

Quantification of MZT2B (A), CDC16 (B) and UBE2T (C) mRNA and protein levels by tumor stage in GC of the diffuse intestinal. *p <0.008, **p <0.001, ***p <0.0002, ****p <0.0001 significant difference between groups (Nonparametric Mann – Whitney test). In all graphs, the expression in gastric tumors was normalized by matched non-neoplastic gastric tissue. RQ: relative quantification; T: tumor sample; N: normal mucosa sample. (D) ROC curve analysis. The cutoff point was chosen as the highest point of the Area-Under-the-Curve (AUC) for MZT2B, AUC: 0.803; (95% CI 0.69-0.82) with 84.2% sensitivity and 50.0% specificity, for CDC16, AUC: 0.798; (95% CI 0.69-0.82) with 86.0% sensitivity and 49.08% specificity and for UBE2T, AUC: 0.549; (95% CI 0.51-0.68) with 52.03% sensitivity and 47.05% specificity. (E) Heat maps showing the average levels of gene expression of ~30 samples of GC tissues of the intestinal-type. The red gradient shows the highest levels of expression while the blue gradient shows the lowest levels. Kaplan-Meier analysis of the overall survival (in months) of patients with gastric cancer of the intestinal-type as a function of the expression of (F) MZT2B, (G) CDC16 and (H) UBE2T. We analyzed high expression (gene expression ≥1.45; red line), as opposed to low expression (gene expression <1.45; blue line) associated with a higher probability of survival. We found more significant values only for high expression of CDC16 (p <0.0085) when associated with the lower shortest patient survival.