Figure 1. Downregulation of SULT2B1b limits proliferation and inflammation in macrophages. (A–C) Transfection of shSULT2B1b reduces SULT2B1b mRNA and protein levels in macrophages. After 24 hours of adenovirus transfection (MOI=400), RAW264.7 cells were treated with ox-LDL (100 mg/ml) for following 24 hours. (A) SULT2B1b mRNA expression was detected by RT-qPCR. Data are represented as the mean±SD (n=3). (B, C) SULT2B1b protein expression was determined by Western blotting. β-actin was used as a reference control. (D, E) IL-6 (D) and TNF-α (E) mRNA expression was determined by RT-qPCR. Data are represented as the mean±SD (n=3). (F–H) The proliferative ability of RAW264.7 cells was measured by EdU staining and CCK8 assay. Representative images and quantification analysis (F, G) of the percentage of EdU-positive cells are shown. Nuclei were stained blue with DAPI. Data are shown as the mean±SD (n=3). **p<0.01, ***p<0.001. ns, no significant difference.