Research Paper Volume 13, Issue 3 pp 3428—3442

Reduced SULT2B1b expression alleviates ox-LDL-induced inflammation by upregulating miR-148-3P via inhibiting the IKKβ/NF-κB pathway in macrophages

Downregulation of SULT2B1b limits proliferation and inflammation in macrophages. (A–C) Transfection of shSULT2B1b reduces SULT2B1b mRNA and protein levels in macrophages. After 24 hours of adenovirus transfection (MOI=400), RAW264.7 cells were treated with ox-LDL (100 mg/ml) for following 24 hours. (A) SULT2B1b mRNA expression was detected by RT-qPCR. Data are represented as the mean±SD (n=3). (B, C) SULT2B1b protein expression was determined by Western blotting. β-actin was used as a reference control. (D, E) IL-6 (D) and TNF-α (E) mRNA expression was determined by RT-qPCR. Data are represented as the mean±SD (n=3). (F–H) The proliferative ability of RAW264.7 cells was measured by EdU staining and CCK8 assay. Representative images and quantification analysis (F, G) of the percentage of EdU-positive cells are shown. Nuclei were stained blue with DAPI. Data are shown as the mean±SD (n=3). **p

Figure 1. Downregulation of SULT2B1b limits proliferation and inflammation in macrophages. (AC) Transfection of shSULT2B1b reduces SULT2B1b mRNA and protein levels in macrophages. After 24 hours of adenovirus transfection (MOI=400), RAW264.7 cells were treated with ox-LDL (100 mg/ml) for following 24 hours. (A) SULT2B1b mRNA expression was detected by RT-qPCR. Data are represented as the mean±SD (n=3). (B, C) SULT2B1b protein expression was determined by Western blotting. β-actin was used as a reference control. (D, E) IL-6 (D) and TNF-α (E) mRNA expression was determined by RT-qPCR. Data are represented as the mean±SD (n=3). (FH) The proliferative ability of RAW264.7 cells was measured by EdU staining and CCK8 assay. Representative images and quantification analysis (F, G) of the percentage of EdU-positive cells are shown. Nuclei were stained blue with DAPI. Data are shown as the mean±SD (n=3). **p<0.01, ***p<0.001. ns, no significant difference.