Research Paper Volume 13, Issue 3 pp 3443—3458

Hypoxia-inducible factor 2-alpha-dependent induction of IL-6 protects the heart from ischemia/reperfusion injury

Effects of HIF2α on cell apoptosis and cell survival of cardiomyocytes during hypoxia. (A) qPCR and Western blot analysis of the efficiency of HIF2α gene- specific downregulation of lentiviral vectors. (B) CCK-8 detection of the effect of downregulation of the HIF2α gene on early cell apoptosis and cell survival. (C) Application of FACS-Annexin V/PI double staining to detect the effect of downregulation of the HIF2α gene on cell apoptosis. n = 3 per group. Data represent the mean ± SEM. **P

Figure 1. Effects of HIF2α on cell apoptosis and cell survival of cardiomyocytes during hypoxia. (A) qPCR and Western blot analysis of the efficiency of HIF2α gene- specific downregulation of lentiviral vectors. (B) CCK-8 detection of the effect of downregulation of the HIF2α gene on early cell apoptosis and cell survival. (C) Application of FACS-Annexin V/PI double staining to detect the effect of downregulation of the HIF2α gene on cell apoptosis. n = 3 per group. Data represent the mean ± SEM. **P<0.01 versus the indicated group.