Figure 4. Changes in the SIRT1/p53/Ac-p53/p21 pathway in postprandial TRL-induced AMSC senescence. (A) AMSCs reached approximately 30%-40% culture-confluence were treated with 0, 25, 50, or 100 μg/mL postprandial TRL for 8 d, and were harvested to detect the protein levels of SIRT1, Ac-p53, p53, and p21 using western blotting. (B–D) The relative protein levels of SIRT1 (B), Ac-p53 (C), and p53 (D) were analyzed using ImageJ. Data are expressed as the mean ± SD (n ≥ 3). *P < 0.05, **P < 0.01 when compared with the control group.