Figure 2. In vitro analysis of osteoblastic differentiation and cell migration of osteoblasts treated with PDB. (A) RT-PCR analysis dependent expression of osteogenic markers (RUNX2, OCN, and OPN). (B) Quantitative analysis of matrix mineralization in MEFs by alizarin Red S staining. (C) Protein expression of migratory and epithelial to mesenchymal transition (EMT) markers potential of MEFs. (D) Quantification analysis of transwell cell migration assay. The experimental group represent PDB-treatment; whereas, no any treatment served as control. The representative results of 3 experiments demonstrated mean ± SD. *P < 0.05.