Figure 5. In vitro analysis of osteoblastic differentiation and cell migration of osteoblasts treated by PDB with and without inhibitor of TGF-β receptor I (Ti) in a concentration of 25nM (Ti25). (A) RT-PCR analysis dependent expression of osteogenic markers (RUNX2, OCN, and OPN). (B) Quantitative analysis of matrix mineralization in MEFs by alizarin Red S staining. (C) Protein expression of migratory and epithelial to mesenchymal transition (EMT) markers potential of MEFs. (D) Quantification analysis of transwell cell migration assay. The representative results of 3 experiments demonstrated mean ± SD. *P < 0.05.