Research Paper Volume 13, Issue 1 pp 1294—1313

Exercise reduces metabolic burden while altering the immune system in aged mice

Analysis of energy metabolism during exercise in young and aged mice. (A–F) Metabolic measurements were performed in young control, young exercise, old control, and old exercise groups (n = 5, respectively) in CLAMS metabolic cages after 4-week of exercise. (A) Kinetic data for VO2 (mg/kg/hr) consumption are shown as mean for each time point in young control (YC; blue circles), young exercise (YE; red rectangles), old control (OC; green triangles) and old exercise (OE, purple reversed triangles) groups. (B) Average VO2 (mg/kg/hr) are shown for total, night (dark) and day (light) cycles. (C) Kinetic data for VCO2 (mg/kg/hr) production are shown as mean for each time point in YC, YE, OC, and OE groups. (D) Average VCO2 (mg/kg/hr) production are shown for total, night (dark) and day (light) cycles. (E) Kinetic data for respiratory exchange ratio (RER) is shown as mean for each time point in YC, YE, OC, and OE groups. (F) Average RER are shown for total, night (dark) and day (light) cycles. (G–H) Average percent body fat (G) and lean mass (H) for YC, YE, OC, and OE were measured using Minispec LF-50. *p p p p

Figure 2. Analysis of energy metabolism during exercise in young and aged mice. (AF) Metabolic measurements were performed in young control, young exercise, old control, and old exercise groups (n = 5, respectively) in CLAMS metabolic cages after 4-week of exercise. (A) Kinetic data for VO2 (mg/kg/hr) consumption are shown as mean for each time point in young control (YC; blue circles), young exercise (YE; red rectangles), old control (OC; green triangles) and old exercise (OE, purple reversed triangles) groups. (B) Average VO2 (mg/kg/hr) are shown for total, night (dark) and day (light) cycles. (C) Kinetic data for VCO2 (mg/kg/hr) production are shown as mean for each time point in YC, YE, OC, and OE groups. (D) Average VCO2 (mg/kg/hr) production are shown for total, night (dark) and day (light) cycles. (E) Kinetic data for respiratory exchange ratio (RER) is shown as mean for each time point in YC, YE, OC, and OE groups. (F) Average RER are shown for total, night (dark) and day (light) cycles. (GH) Average percent body fat (G) and lean mass (H) for YC, YE, OC, and OE were measured using Minispec LF-50. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.