Research Paper Volume 13, Issue 1 pp 1332—1356

CCDC170 affects breast cancer apoptosis through IRE1 pathway

CCDC170 promoted cell apoptosis in MCF7 breast cancer cells. (A, C) Representative images of flow cytometry (FCM) using Annexin V-FITC and PI staining. Column bar graph showing a dramatically bigger early and late apoptosis ratio in CCDC170-transiently-overexpressing MCF7 cells than the control cells (B). The cell apoptosis ratio was significantly lower in the cells with CCDC170 knockdown compared with the control cells (D). Each group was independently repeated three times, 3000 cells were calculated. (E) Representative images were taken with nuclear stain DAPI (blue) and apoptosis stain TUNEL (green). (F) The result depicts the percentage of TUNEL positive nuclei of MCF-7 cells after CCDC170 upregulation. Scale bar, 50 μm. (G, I) Representative western blot bands of Caspase12 in MCF7 cells with CCDC170 up-regulated transiently (H) and stably (J). pCMV-CCDC170(control) represented CCDC170-transiently-overexpressing MCF7 cells and controls. v-CCDC170(control) represented CCDC170-stably-overexpressing MCF7 cells and controls. β-actin was used as a reference for calculating the relative protein expression. The error bars presented as mean ± Standard Error of Mean (SEM) with analysis of unpaired Student’s t-test. *P

Figure 4. CCDC170 promoted cell apoptosis in MCF7 breast cancer cells. (A, C) Representative images of flow cytometry (FCM) using Annexin V-FITC and PI staining. Column bar graph showing a dramatically bigger early and late apoptosis ratio in CCDC170-transiently-overexpressing MCF7 cells than the control cells (B). The cell apoptosis ratio was significantly lower in the cells with CCDC170 knockdown compared with the control cells (D). Each group was independently repeated three times, 3000 cells were calculated. (E) Representative images were taken with nuclear stain DAPI (blue) and apoptosis stain TUNEL (green). (F) The result depicts the percentage of TUNEL positive nuclei of MCF-7 cells after CCDC170 upregulation. Scale bar, 50 μm. (G, I) Representative western blot bands of Caspase12 in MCF7 cells with CCDC170 up-regulated transiently (H) and stably (J). pCMV-CCDC170(control) represented CCDC170-transiently-overexpressing MCF7 cells and controls. v-CCDC170(control) represented CCDC170-stably-overexpressing MCF7 cells and controls. β-actin was used as a reference for calculating the relative protein expression. The error bars presented as mean ± Standard Error of Mean (SEM) with analysis of unpaired Student’s t-test. *P < 0.05, compared with control group.