Research Paper Volume 13, Issue 1 pp 104—133

Dietary and environmental factors have opposite AhR-dependent effects on C. elegans healthspan

AHR-1 displays evolutionarily conserved functions and affects aging in a diet-dependent manner. (A, B) ahr-1 is more sensitive to xenobiotic stress. Pooled lifespan/healthspan curves of 120 worms/condition in 2 independent replicates treated with either DMSO or 5 μM BaP from adulthood are shown. (C, D) ahr-1 is more sensitive to UVB stress. Pooled lifespan/healthspan curves of 300 (wt control, ahr-1 control), 180 (wt UVB) and 178 (ahr-1 UVB) worms/condition in 3 independent replicates either left untreated or treated with 1200 J/m² UVB from adulthood are shown. (E, F) AHR-1 affects aging in a diet-dependent manner. Pooled lifespan/healthspan curves of 170 (wt OP50) and 180 (all other conditions) worms/condition in 3 independent replicates grown either on HT115 or OP50 are shown. (A–F) Statistical test: Log-Rank test, # significance vs. control/HT115, * significance vs. wt, p-value

Figure 2. AHR-1 displays evolutionarily conserved functions and affects aging in a diet-dependent manner. (A, B) ahr-1 is more sensitive to xenobiotic stress. Pooled lifespan/healthspan curves of 120 worms/condition in 2 independent replicates treated with either DMSO or 5 μM BaP from adulthood are shown. (C, D) ahr-1 is more sensitive to UVB stress. Pooled lifespan/healthspan curves of 300 (wt control, ahr-1 control), 180 (wt UVB) and 178 (ahr-1 UVB) worms/condition in 3 independent replicates either left untreated or treated with 1200 J/m² UVB from adulthood are shown. (E, F) AHR-1 affects aging in a diet-dependent manner. Pooled lifespan/healthspan curves of 170 (wt OP50) and 180 (all other conditions) worms/condition in 3 independent replicates grown either on HT115 or OP50 are shown. (AF) Statistical test: Log-Rank test, # significance vs. control/HT115, * significance vs. wt, p-value < 0.05.