Figure 3. HOXB9 overexpression promotes migration and invasion, but not proliferation, in A549 cells. (A) A vector encoding Flag-HOXB9 was used to induce HOXB9 overexpression in A549 cells. An empty vector served as control. HOXB9 overexpression was confirmed by western blot and gray level analysis. (B) CCK-8 assay results showing no significant differences in cell proliferation between Flag-HOXB9 and control A549 cells. (C) Wound-healing closure images of Flag-HOXB9 and control A549 cells. (D) Quantification of results from wound-healing assays shown in (C); p = 0.0007. (E) Transwell assays comparing invasion potential between control and HOXB9-overexpressing A549 cells. (F) Quantification of results from experiments shown in (E); p = 0.0238.