Figure 4. High-salt intake induces NFAT5 nuclear translocation in ECs. (A) En face immunofluorescent staining of NFAT5 (red) in ECs of TA and AA regions of ApoE-/- mice in normal and high salt groups after 4 weeks feeding. Nuclei were stained by DAPI. NFAT5 nuclear translocation in ECs was marked by circles. (B) Protein expression of NFAT5 in HUVECs that exposed to different hyper-osmotic media for three days. All data were presented as mean ± SEM, N≥3. *p < 0.05 versus control group.