Research Paper Volume 13, Issue 3 pp 3680—3698

Polyglutamine-expanded ataxin3 alter specific gene expressions through changing DNA methylation status in SCA3/MJD

DNA methylation status of sequence in En1, Nkx2-1, and Otx1 and the corresponding transcriptional levels of these genes. (A) Compared to control group, the average methylation levels of sequence En1

Figure 3. DNA methylation status of sequence in En1, Nkx2-1, and Otx1 and the corresponding transcriptional levels of these genes. (A) Compared to control group, the average methylation levels of sequence En1_2, En1_3, and Otx1 were higher in SCA3/MJD mice (p<0.05). While the average methylation levels of sequences Nkx2-1_2 and Nkx2-1_3 were lower in SCA3/MJD mice (p<0.05). (B) the transcriptional levels of En1, Nkx2-1 and Otx1 were significantly different between SCA3/MJD and control groups (p<0.05). No significantly differences were detected in the other genes.