Research Paper Volume 13, Issue 3 pp 3680—3698

Polyglutamine-expanded ataxin3 alter specific gene expressions through changing DNA methylation status in SCA3/MJD

Dynamic changes of En1, Nkx2-1, and Otx1 protein levels in 12-week and 19-month old mice cerebellum. Compared to the WT group, the protein level of En1 showed a significant downregulation in SCA3/MJD group at both 12 weeks and 19 months (pppp>0.05).

Figure 6. Dynamic changes of En1, Nkx2-1, and Otx1 protein levels in 12-week and 19-month old mice cerebellum. Compared to the WT group, the protein level of En1 showed a significant downregulation in SCA3/MJD group at both 12 weeks and 19 months (p<0.05). And at 19 months of age, the protein level of En1 was higher than that at 12 weeks in both WT and SCA3/MJD mice cerebellum (p<0.05). For Nkx2-1, the protein levels were significantly upregulated in the SCA3/MJD group than in WT group (p<0.05). No significant difference was observed for the Otx1 protein level between the SCA3/MJD and WT group at both 12 weeks and 19 months of age (p>0.05).