Research Paper Advance Articles

SphK1-targeted miR-6784 inhibits functions of skin squamous cell carcinoma cells

miR-6784 overexpression induces apoptosis activation in skin SCC cells. A431 cells (A–F), primary skin SCC cells (“C1”/“C2”, G, H), and SCC-9 cells (G, H) expressing the lentiviral construct encoding pre-miR-6784 (“OE-miR-6784”) or the nonsense control microRNA sequence (“miRC”) were established. Cells were cultured for applied time periods, caspase and apoptosis activation were tested by mentioned appropriate assays. Data were presented as mean ± standard deviation (SD, n=5). Experiments in this study were repeated three times with similar results obtained. *pvs. “miRC” cells.

Figure 3. miR-6784 overexpression induces apoptosis activation in skin SCC cells. A431 cells (AF), primary skin SCC cells (“C1”/“C2”, G, H), and SCC-9 cells (G, H) expressing the lentiviral construct encoding pre-miR-6784 (“OE-miR-6784”) or the nonsense control microRNA sequence (“miRC”) were established. Cells were cultured for applied time periods, caspase and apoptosis activation were tested by mentioned appropriate assays. Data were presented as mean ± standard deviation (SD, n=5). Experiments in this study were repeated three times with similar results obtained. *p< 0.05 vs. “miRC” cells.