Research Paper Volume 13, Issue 3 pp 3726—3741

SphK1-targeted miR-6784 inhibits functions of skin squamous cell carcinoma cells

miR-6784 inhibition induces SphK1 elevation and promotes A431 cell progression. A431 cells, expressing the lentiviral construct encoding the pre-miR-6784 anti-sense (“antagomiR-6784-sL1/sL2”, two stable cell lines) or the control nonsense microRNA anti-sense (“antaC”), were established. Cells were cultured for applied time periods. Expression miR-6784, SphK1 and SphK2 (A–D) was tested; Cellular ceramide contents were shown (E); Cell viability (CCK-8 OD, F), proliferation (by recording EdU-positive nuclei ratio, G), migration (“Transwell” assay, H), and invasion (“Matrigel Transwell” assay, I) were tested. Data were presented as mean ± standard deviation (SD, n=5). Experiments in this study were repeated three times with similar results obtained. *pvs. “antaC” cells. Scale bar=100 μm (G–I).

Figure 4. miR-6784 inhibition induces SphK1 elevation and promotes A431 cell progression. A431 cells, expressing the lentiviral construct encoding the pre-miR-6784 anti-sense (“antagomiR-6784-sL1/sL2”, two stable cell lines) or the control nonsense microRNA anti-sense (“antaC”), were established. Cells were cultured for applied time periods. Expression miR-6784, SphK1 and SphK2 (AD) was tested; Cellular ceramide contents were shown (E); Cell viability (CCK-8 OD, F), proliferation (by recording EdU-positive nuclei ratio, G), migration (“Transwell” assay, H), and invasion (“Matrigel Transwell” assay, I) were tested. Data were presented as mean ± standard deviation (SD, n=5). Experiments in this study were repeated three times with similar results obtained. *p< 0.05 vs. “antaC” cells. Scale bar=100 μm (GI).