Research Paper Volume 13, Issue 3 pp 3763—3778

Dl-3-n-butylphthalide inhibits neuroinflammation by stimulating foxp3 and Ki-67 in an ischemic stroke model

NBP inhibits NLRP3 inflammasome activation in the right striatum of the rat brain following pMCAO. (A) Representative NLRP3, Caspase-1 and IL-1β immunohistochemistry results are shown for the right striatum on the lesioned side at 200× magnification. Scale bar = 3 mm for the full coronal section; 100 μm for microscopic observation. (B) The score was calculated based on the percentage contribution of positive expression using IHC

Figure 3. NBP inhibits NLRP3 inflammasome activation in the right striatum of the rat brain following pMCAO. (A) Representative NLRP3, Caspase-1 and IL-1β immunohistochemistry results are shown for the right striatum on the lesioned side at 200× magnification. Scale bar = 3 mm for the full coronal section; 100 μm for microscopic observation. (B) The score was calculated based on the percentage contribution of positive expression using IHC_Profiler in Image J. The data are presented as the mean ± SD, and were assessed using one-way ANOVA. # P < 0.05 vs. the sham group, ## P < 0.01 vs. the sham group, ### P < 0.001 vs. the sham group, * P < 0.05 vs. the pMCAO group, ** P < 0.01 vs. the pMCAO group, *** P < 0.001 vs. the pMCAO group, n = 3. Molecular docking of NBP to (C) NLRP3, (D) IL-1β, (E) Caspase-1.