Figure 1. The identification and purity of primary microglia. Primary microglial cells were isolated from one-day-old SD rats and grown in DMEM/F12 medium. The identification and purity of microglial cells was determined by immunofluorescent staining with CD11b (Red). To distinguish microglia from macrophages, the double staining for CD11b and CD45 (Green) were performed. In merged images, all the CD11b-positive cells (Red) almost coincide with all the DAPI-positive cells (Blue). Scale bar: 20 μm. DAPI: 4',6-diamidino-2-phenylindole.