Research Paper Volume 12, Issue 24 pp 26121—26139

Subanesthetic isoflurane abates ROS-activated MAPK/NF-κB signaling to repress ischemia-induced microglia inflammation and brain injury

Figure 1. The identification and purity of primary microglia. Primary microglial cells were isolated from one-day-old SD rats and grown in DMEM/F12 medium. The identification and purity of microglial cells was determined by immunofluorescent staining with CD11b (Red). To distinguish microglia from macrophages, the double staining for CD11b and CD45 (Green) were performed. In merged images, all the CD11b-positive cells (Red) almost coincide with all the DAPI-positive cells (Blue). Scale bar: 20 μm. DAPI: 4',6-diamidino-2-phenylindole.