Research Paper Volume 13, Issue 3 pp 3866—3885

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer

LINC00485 is downregulated in CRC tissues and cells. (A) LINC00485 levels in CRC and adjacent normal tissues. (B) LINC00485 levels in CRC patients with stage I/II or III/IV disease is significantly lower than in adjacent normal tissues. (C) High expression of LINC00485 predicts a favorable prognosis of CRC patients. (D) The subcellular localization of LINC00485 in SW480 and LoVo cells was detected by FISH assay. Scale bar, 2 μm. (E) LINC00485 expression is significantly reduced in CRC cells compared to human normal colorectal epithelial cell lines. (F) The knockdown efficiency of LINC00485 RNAi lentivirus in FHC cells. (G) LINC00485 knockdown promotes cell migration in FHC cells. (H) LINC00485 knockdown promotes the migration of FHC cells. (I) Overexpression of LINC00485 suppresses the migratory ability of LoVo cells. Differences between two groups were assessed by applying student’s t-test. Multiple comparison was analyzed using the one-way ANOVA with LSD test. Bars were represented as S.D. *PPPLINC00485.

Figure 1. LINC00485 is downregulated in CRC tissues and cells. (A) LINC00485 levels in CRC and adjacent normal tissues. (B) LINC00485 levels in CRC patients with stage I/II or III/IV disease is significantly lower than in adjacent normal tissues. (C) High expression of LINC00485 predicts a favorable prognosis of CRC patients. (D) The subcellular localization of LINC00485 in SW480 and LoVo cells was detected by FISH assay. Scale bar, 2 μm. (E) LINC00485 expression is significantly reduced in CRC cells compared to human normal colorectal epithelial cell lines. (F) The knockdown efficiency of LINC00485 RNAi lentivirus in FHC cells. (G) LINC00485 knockdown promotes cell migration in FHC cells. (H) LINC00485 knockdown promotes the migration of FHC cells. (I) Overexpression of LINC00485 suppresses the migratory ability of LoVo cells. Differences between two groups were assessed by applying student’s t-test. Multiple comparison was analyzed using the one-way ANOVA with LSD test. Bars were represented as S.D. *P<0.05; **P<0.01; ***P<0.001. N, normal tissues; sh, short hairpin RNA targeting LINC00485.