Research Paper Volume 13, Issue 3 pp 3886—3897

Praja2 suppresses the growth of gastric cancer by ubiquitylation of KSR1 and inhibiting MEK-ERK signal pathways


Figure 3. Overexpression of KSR1 promoted the progression of GC cells. (A, B) The transfection efficiency of KSR1 in MKN-45 cells using qRT-PCR and western blot. (C) CCK8 for viability of MKN-45 cells. (D) Edu assay for proliferation of MKN-45 cells. Scale bar, 40 μm. (E) Wound healing assay for migration of MKN-45 cells. Scale bar, 60 μm. (F) Transwell assay for invasion of MKN-45 cells. Scale bar, 60 μm. (G) Flow cytometry assay for apoptosis of MKN-45 cells. Data are mean ± SD; *P < 0.05.