Research Paper Volume 13, Issue 3 pp 3909—3925

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression

Enrichment of FTO protein but not mRNA in BLCA. (A) Heatmap showing expression of the major m6A methylases and demethylases in BLCA (T) and normal tissues (N). (B) Comparison of FTO mRNA levels between BLCA tumors (red box) and normal tissues (grey box). (C) Overall survival in BLCA patients with the higher FTO level was shorter as demonstrated by Kaplan-Meier analysis. (D) FTO transcript in patient-derived bladder tumor (T) and normal tissues (N) by qPCR. * p t-test. (E) FTO protein expression in patient-derived normal tissues (N) and BLCA tumor (T) by western blot. * p t-test. (F) FTO mRNA expression in BLCA and normal uroepithelial SV-HUC-1 cells by qPCR. (G) FTO protein profiling in BLCA and normal uroepithelial cell lines by western blot.

Figure 1. Enrichment of FTO protein but not mRNA in BLCA. (A) Heatmap showing expression of the major m6A methylases and demethylases in BLCA (T) and normal tissues (N). (B) Comparison of FTO mRNA levels between BLCA tumors (red box) and normal tissues (grey box). (C) Overall survival in BLCA patients with the higher FTO level was shorter as demonstrated by Kaplan-Meier analysis. (D) FTO transcript in patient-derived bladder tumor (T) and normal tissues (N) by qPCR. * p < 0.05, student’s t-test. (E) FTO protein expression in patient-derived normal tissues (N) and BLCA tumor (T) by western blot. * p < 0.05, student’s t-test. (F) FTO mRNA expression in BLCA and normal uroepithelial SV-HUC-1 cells by qPCR. (G) FTO protein profiling in BLCA and normal uroepithelial cell lines by western blot.