Research Paper Volume 13, Issue 3 pp 3909—3925

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression

Turn down USP18 or FTO inhibits BLCA cell proliferation and migration. (A) Time-dependent BLCA cell proliferation upon depletion of USP18 by MTT assay. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). ** p t-test. (B) Wound-healing assay assessing the role of USP18 in BLCA cell migration. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). (C) MTT assay assessing time-dependent BLCA cell proliferation upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression. *p t-test. (D) Wound-healing assay assessing BLCA cell migration upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression.

Figure 4. Turn down USP18 or FTO inhibits BLCA cell proliferation and migration. (A) Time-dependent BLCA cell proliferation upon depletion of USP18 by MTT assay. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). ** p < 0.01, *** p < 0.001 vs. control, student’s t-test. (B) Wound-healing assay assessing the role of USP18 in BLCA cell migration. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). (C) MTT assay assessing time-dependent BLCA cell proliferation upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression. *p < 0.05, **p < 0.01 vs. control, student’s t-test. (D) Wound-healing assay assessing BLCA cell migration upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression.