Figure 4. Proteomic differences between high-risk and low-risk patients; the importance of the cytoskeleton reflected in levels of neutrophil degranulation, platelet degranulation and endomembrane trafficking proteins. (A) Hierarchical clustering of 33 patients was based on the expression (SILAC log2 ratio) of 205 proteins with significantly different regulation in AML cells from high-risk (dark grey squares) and low-risk patients (light grey squares). Two vertical main clusters were observed, one dominated by proteins with higher abundance in mostly high-risk patients (upper cluster) and the other by proteins with higher abundance in low-risk patients (lower cluster). GO and KEGG pathways analyses of the two protein clusters were performed to reveal enriched BP, CC and MF terms for the high-risk and low-risk patients. The various enriched GO terms and KEGG pathways are displayed in the scatter plot. The number of genes associated to a specific GO term or KEGG pathway (count) and the corresponding –log10P values are shown on the x-axis and y-axis, respectively. Abbreviations were used in cases of long GO term or KEGG pathway name (Pathogen. for Pathogenic; fil. for filament). (B) Reactome term enrichment was performed using the STRING app (1.5.1) in Cytoscape. The five Reactome pathways with highest significance are shown with the corresponding FDR values. The protein nodes are colored according to their high-risk/low-risk FC, i.e. orange indicates increased abundance in the high-risk group and blue increased abundance in the low-risk group.