Research Paper Volume 13, Issue 3 pp 3945—3956

MicroRNA-205-5p targets the HOXD9-Snail1 axis to inhibit triple negative breast cancer cell proliferation and chemoresistance

miR-205 regulates Snail1 expression to mediate breast cancer cell proliferation and chemotherapy resistance. (A) Verifying the correlation between miR-205 and Snail1 mRNA in 100 TNBC specimens. (B) The correlation between miR-205 and Snail1 protein expression was calculated in 100 TNBC specimens. (C) Western blot analysis of the Snail1 protein and mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (D) qRT-PCR analysis of the Snail1 protein and mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (E) Western blot are used to screen the Snail1 siRNA sequences with the best interference effect for subsequent experiments. (F) qRT-PCR are used to screen the Snail1 siRNA sequences with the best interference effect for subsequent experiments. (G) CCK8 was used to analyze the Snail1 knockdown effect with the miR-205 knockdown treated BT-549 cells and miR-205 overexpression treated MDA-MB-231 cells. (H) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with Cisplatin treatment. (I) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with Doxorubicin treatment. (J) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with three Paclitaxel treatment. (K) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Cisplatin treatment. (L) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Doxorubicin treatment. (M) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Paclitaxel treatment. ***P

Figure 4. miR-205 regulates Snail1 expression to mediate breast cancer cell proliferation and chemotherapy resistance. (A) Verifying the correlation between miR-205 and Snail1 mRNA in 100 TNBC specimens. (B) The correlation between miR-205 and Snail1 protein expression was calculated in 100 TNBC specimens. (C) Western blot analysis of the Snail1 protein and mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (D) qRT-PCR analysis of the Snail1 protein and mRNA expression in miR-205 knockdown treatment in BT-549 cells and miR-205 overexpression treatment in MDA-MB-231 cells. (E) Western blot are used to screen the Snail1 siRNA sequences with the best interference effect for subsequent experiments. (F) qRT-PCR are used to screen the Snail1 siRNA sequences with the best interference effect for subsequent experiments. (G) CCK8 was used to analyze the Snail1 knockdown effect with the miR-205 knockdown treated BT-549 cells and miR-205 overexpression treated MDA-MB-231 cells. (H) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with Cisplatin treatment. (I) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with Doxorubicin treatment. (J) Cell proliferation analysis of the miR-205 overexpression and Snail1 knockdown treated MDA-MB-231 cells with three Paclitaxel treatment. (K) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Cisplatin treatment. (L) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Doxorubicin treatment. (M) Cell proliferation analysis of the miR-205 and Snail1 knockdown treated BT-549 cells with Paclitaxel treatment. ***P<0.001.