Research Paper Volume 13, Issue 3 pp 4045—4062

ALKBH5-mediated m6A demethylation of FOXM1 mRNA promotes progression of uveal melanoma

ALKBH5 knockdown inhibited UM cell growth. (A) Knockdown of ALKBH5 suppressed cell proliferation in UM cells as determined by MTT assay. (B) ALKBH5 shRNA treatment induced cell cycle arrest in G1/S phase in UM cells. (C) ALKBH5 elicited an apoptotic response in UM cells as determined by annexin V-PITC and PI staining and flow cytometry. (D) Western blot showed increased expression of apoptotic related protein, cleaved caspase-3, and decreased expression of BCL-2, c-Myc, cyclin D1 and cyclin E1 in ALKBH5-knockdown UM cells. Mean ± SEM, t-test, *P P P

Figure 3. ALKBH5 knockdown inhibited UM cell growth. (A) Knockdown of ALKBH5 suppressed cell proliferation in UM cells as determined by MTT assay. (B) ALKBH5 shRNA treatment induced cell cycle arrest in G1/S phase in UM cells. (C) ALKBH5 elicited an apoptotic response in UM cells as determined by annexin V-PITC and PI staining and flow cytometry. (D) Western blot showed increased expression of apoptotic related protein, cleaved caspase-3, and decreased expression of BCL-2, c-Myc, cyclin D1 and cyclin E1 in ALKBH5-knockdown UM cells. Mean ± SEM, t-test, *P < 0.05, **P < 0.01, ***P < 0.001.