Research Paper Volume 13, Issue 3 pp 4045—4062

Figure 5. ALKBH5 increases FOXM1 expression and FOXM1 mRNA stability in UM cells. (A) The protein levels of FOXM1 in wild-type or catalytic inactive mutation ALKBH5-expressing UM cells were measured using western blotting. (B) ALKBH5 downregulation decreased FOXM1 expression in UM cells. (C) MeRIP-qPCR analysis was used to verify ALKBH5-induced FOXM1 m⁶A modification. The m⁶A modification of FOXM1 was increased on downregulation and catalytic inactive mutation of ALKBH5 in C918 cells. (D) C918 cells were treated with dactinomycin (Act D, 2 μg/mL) to block new RNA synthesis. The stability of FOXM1 was measured by qRT-PCR at different times. Mean ± SEM, t-test, *P < 0.05, **P < 0.01, ***P < 0.001.